Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6554145 | Forensic Science International: Genetics | 2014 | 7 Pages |
Abstract
Here, we describe the application of a data generation and analysis workflow to the development of more than 400 complete, forensic-quality mtGenomes from low DNA quantity blood serum specimens as part of a U.S. National Institute of Justice funded reference population databasing initiative. We discuss the minor modifications made to a published mtGenome Sanger sequencing protocol to maintain a high rate of throughput while minimizing manual reprocessing with these low template samples. The successful use of this semi-automated strategy on forensic-like samples provides practical insight into the feasibility of producing complete mtGenome data in a routine casework environment, and demonstrates that large (>2Â kb) mtDNA fragments can regularly be recovered from high quality but very low DNA quantity specimens. Further, the detailed empirical data we provide on the amplification success rates across a range of DNA input quantities will be useful moving forward as PCR-based strategies for mtDNA enrichment are considered for targeted next-generation sequencing workflows.
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Authors
Rebecca S. Just, Melissa K. Scheible, Spence A. Fast, Kimberly Sturk-Andreaggi, Jennifer L. Higginbotham, Elizabeth A. Lyons, Jocelyn M. Bush, Michelle A. Peck, Joseph D. Ring, Toni M. Diegoli, Alexander W. Röck, Gabriela E. Huber, Simone Nagl,