Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6554197 | Forensic Science International: Genetics | 2014 | 4 Pages |
Abstract
Insertion/deletion (INDELs) marker sets can serve as a useful supplementary tool for human identification. A commercial kit, the Qiagen DIPplex® Investigator kit, multiplexes 30 biallelic autosomal INDELs and Amelogenin for forensic use. We performed a validation study based on the DIPplex® kit in four Chinese populations: Han, Tibetan, Uyghur, and Kazakh. There were no significant departures from Hardy-Weinberg equilibrium or significant linkage disequilibrium (pair-wised r2 < 0.2) between the 30 INDELs. The random match probabilities were in the range of 3.84 Ã 10â11 to 1.20 Ã 10â12, and the power of exclusion was >0.99. The multiplex PCR was optimized for a 5-μL volume, full profiles were obtained with 0.062 ng/μL of template DNA, and excellent performance was obtained with degraded casework samples. This study demonstrates that the multiplex INDEL assay can be used as a supplementary method for degraded DNA detection in the studied Chinese populations.
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Authors
Yi-Liang Wei, Cui-Jiao Qin, Hui Dong, Jing Jia, Cai-Xia Li,