Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6555314 | Legal Medicine | 2018 | 4 Pages |
Abstract
Previously, an age-predictive method based on DNA-methylation patterns in semen was developed, using three CpG sites (cg06304190 in the TTC7B gene, cg12837463, and cg06979108 in the NOX4 gene). Before considering the routine use of a new method in forensics, validation studies such as concordance and sensitivity tests are essential for obtaining expanded and more reliable forensic information. Here, we evaluated a previously described age-predictive method for semen for routine forensic use. Concordance testing showed a high correlation between the predicted and chronological age, with a mean absolute deviation from the chronological age of 4.8â¯years. Sensitivity testing suggested that age prediction with reliable accuracy and consistency was possible with >5â¯ng of bisulfite-converted DNA. We also confirmed the applicability of the age-predictive method in forensic casework, using forensic samples. Thus, the proposed method could serve as a very valuable forensics tool for accurate age prediction with semen samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jee Won Lee, Chong Min Choung, Ju Yeon Jung, Hwan Young Lee, Si-Keun Lim,