Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6593245 | Chinese Journal of Chemical Engineering | 2017 | 19 Pages |
Abstract
Some proteins secreted by microorganisms have large molecular weights. We report here an approach to prepare coating by multilayer polymers for antifouling of proteins, especially the proteins with a large molecular weight. Stainless steel was used as the model substrate. The substrate was first coated with a hybrid polymer film, which was formed by simultaneous hydrolytic polycondensation of 3-aminopropyltriethoxysilane and polymerization of dopamine (HPAPD). After grafting the macroinitiator 2-bromoisobutyryl bromide, the block polymer brushes PMMA-b-PHEMA were grafted. Three proteins were used to test protein adsorption and antifouling behavior of the coating, including recombinant green fluorescent (54Â kDa), recombinant R-transaminase (2Â ÃÂ 90Â kDa), and recombinant catalase (4Â ÃÂ 98Â kDa). It is demonstrated that the block polymer brushes not only can prevent the adsorption of small molecular weight proteins, but also can significantly reduce the adsorption of the large molecular weight proteins.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Xianling Meng, Xia Jiang, Peijun Ji,