Modelling the effect of temperature on the gel-filtration chromatographic protein separation

In this study a mechanistic model for chromatography was taken and the solution to its Laplace transfer function was obtained using the Fast Fourier Transform method. Using previously developed correlations for modelling diffusion, both in solution and intraparticle, and estimating the mass transfer coefficient, the effect of temperature in gel fittration liquid chromatography was investigated. The effect of each individual parameter on the elution curve was systematically explored allowing for reasonable estimates for the different temperature cases. In this case, a system of bovine serum albumin and phenylalanine separated by gel filtration chromatography was simulated to demonstrate how the resolution and the selectivity of the separation will change with physical parameters. Decreasing the particle size and flow rate while increasing the temperature led to higher resolution, which is consistent with experimental literature data.