Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6606817 | Electrochimica Acta | 2016 | 9 Pages |
Abstract
We report on the design of electrochemical DNA-biosensors made from 15, 25 and 50 μm diameter gold ultramicroelectrodes (UMEs) in a two-electrode setup. The biosensors were prepared by chemical adsorption of 22-base thiol-labeled DNA-probes in a self-assembled monolayer configuration onto the surface of the gold UME. Hybridization events were transduced by FeIII/FeII redox process electro-mediated by DNA-redox intercalated mediators. The optimization of the DNA-biosensors was achieved by using different FeIII/FeII ligands (cyanide, cefadroxil, deferoxamine) and intercalators (methylene blue, proflavine). Optimized biosensors allowed direct and selective amperometric detection in the femtomolar range and of single mismatch within a 35 minutes protocol.
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Authors
A. Nassi, F.-X. Guillon, A. Amar, B. Hainque, S. Amriche, D. Maugé, E. Markova, C. Tsé, P. Bigey, M. Lazerges, F. Bedioui,