Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6608183 | Electrochimica Acta | 2016 | 24 Pages |
Abstract
Hydrogen peroxide is a dominating coreactant of most luminol electrochemiluminescence (ECL) studies though it is unstable and sensitive to many metal ions. Tripropylamine is an eminent coreactant of tris(2,2'-bipyridine)ruthenium ECL. In comparison with H2O2, tripropylamine is much more stable. In this study, tripropylamine has been exploited as a new coreactant of luminol ECL for the first time. The emission spectrum reveals that the ECL peak was aroused by excited 3-aminophthalate. Under optimized conditions, a biphasic linear relationship is obtained between the ECL intensities and luminol concentrations in the presence of 10Â mM tripropylamine over the range of 1.0Â ÃÂ 10â11 â1.0Â ÃÂ 10â7Â M and 1.0Â ÃÂ 10â7 â1.0Â ÃÂ 10â4Â M with a detection limit of 6.9Â ÃÂ 10â12Â M. The detection of tripropylamine showed a linear relationship between ECL intensities and tripropylamine concentrations in the range of 1.0Â ÃÂ 10â6 to 1.0Â ÃÂ 10â2Â M with a detection limit of 7.7Â ÃÂ 10â7Â M. Compared to luminol-H2O2 ECL, luminol-tripropylamine ECL system displays not only high stability but also high sensitivity. Hence, it shows great potential in many fields including immunoassay or non-immunoassay diagnostic, clinical, pharmaceutical, environmental and food analysis in the future.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Saima Hanif, Shuang Han, Peter John, Wenyue Gao, Shimeles Addisu Kitte, Guobao Xu,