Article ID Journal Published Year Pages File Type
660821 International Journal of Heat and Mass Transfer 2008 8 Pages PDF
Abstract

Three-dimensional behavior of ice crystals and cells during freezing and thawing of biological tissues was investigated microscopically in real time by using a confocal laser scanning microscope (CLSM) and a fluorescent dye. Fresh white meat of chicken was stained in physiological saline, and then frozen and thawed under two different thermal protocols. The CLSM noninvasively produced tomograms of the tissues to clarify the pattern of freezing, the morphology of ice crystals in the tissues, and the interaction between ice crystals and cells. The results were compared and correlated with observed histological changes in the post-thaw tissues.

Related Topics
Physical Sciences and Engineering Chemical Engineering Fluid Flow and Transfer Processes
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