Bioelectrochemical studies based on enzyme-electrocatalysis

A mediated amperometric enzyme electrode, which is constructed by immobilizing an enzyme behind a semipermeable membrane on the surface of a carbon paste electrode containing a mediator compound, has allowed a variety of bioelectrochemical studies in both applied and fundamental aspects such as biosensors, biofuel cells, and enzyme kinetics. This paper describes such bioelectrochemical studies in which we have been involved in these 40 years.