Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6661785 | Journal of Electroanalytical Chemistry | 2018 | 6 Pages |
Abstract
10-Hydroxycamptothecine (10-HCPT), a natural alkaloid, is widely used in clinical practice because of its significant anti-cancer activity and small toxic side effects. In this work, graphene nanosheets (GNs) were prepared by ultrasonic exfoliation of graphite using N-methylpyrrolidone (NMP) as the solvent. The prepared GNs were characterized using transmission electron microscopy and scanning electron microscopy, and then used to construct a novel sensing platform for 10-HCPT. The electrochemical behaviors of 10-HCPT on the glassy carbon electrode (GCE) and GNs modified GCE (GNs/GCE) were studied. Compared with bare GCE, the prepared GNs/GCE increased the oxidation signal of 10-HCPT significantly, owing to larger active area of GNs/GCE and thus higher accumulation efficiency of 10-HCPT. Utilizing the signal amplification strategy of GNs, a highly-sensitive electrochemical method was established for the detection of 10-HCPT. The linear range was from 10.0 to 250.0â¯Î¼gâ¯Lâ1, and the detection limit was 3.21â¯Î¼gâ¯Lâ1 (8.81â¯nM). It was used to detect 10-HCPT in urine samples, and satisfactory results were obtained.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Xiaoxia Ye, Xiaofeng Yang, Piaopiao Wei, Kangbing Wu,