Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6662210 | Journal of Electroanalytical Chemistry | 2018 | 23 Pages |
Abstract
Herein, we presented a “signal-on” switch electrochemiluminescence (ECL) biosensor based on molecular recognition and nanocomposite to track circulating tumor cells (CTCs) in real time. CTCs are well-known biomarkers for metastasis. To construct the ECL biosensor for CTC detection, the surface of a glassy carbon electrode (GCE) was first modified with the composite of Ru(bpy)32 +/β-cyclodextrin-Au nanoparticles(β-CD-AuNPs)/graphene, followed by immobilizing ferrocene-labeled aptamers (TLS1c and TLS11a in 1:1 ratio) as the probes (Fc-aptamers/Ru(bpy)32 +/β-cyclodextrin-Au nanoparticles(β-CD-AuNPs)/graphene). In presence of the CTCs, the quencher (Fc) left the electrode after connecting with aptamers, thus enhancing ECL signal, i.e., recovering the “signal-on”. This newly developed ECL biosensor demonstrated supersensitive, selective, rapid detection of the CTCs with a detection limit of 40 cells mLâ 1. In addition, the biosensor exhibited excellent reusability with at least 6 cycles of recovering the original signal. Therefore, this aptamer based “signal-on” switch electrochemiluminescence (ECL) biosensor may help in assessing the prognosis of patients with cancer metastasis.
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Authors
Qian Kun, Yan Lin, Hong Peng, Lin Cheng, HanFeng Cui, Nian Hong, Jun Xiong, Hao Fan,