Supercritical fluid immobilization of horseradish peroxidase on high surface area mesoporous activated carbon

- 54% Higher loading is achieved in scCO2 in 3 h compared to phosphate buffer in 30 h.
- HRP loading in scCO2 is higher for all HRP: carbon ratios used.
- Successfully employed in removing phenol from water in the presence of H2O2.
- Immobilized HRP is more thermally stable than free HRP.
- Immobilized HRP retained enzymatic activity after 11 cycles.