Producing of Microalgal Lipid by Isolated Microalgae under Photoautotrophic and Heterotrophic Cultivations

The objective of this work is to produce lipid from isolated freshwater microalgae strains. Under photoautotrophic cultivation, a biomass of 1.67 g/L with lipid of 23.94 mg/L, 1.2 g/L with lipid of 37.0 mg/L, 1.13 g/L with lipid of 36.4 mg/L were obtained from microalgae isolate Chlorella sp. MSU1, Chlorella sp. MSU2 and Chlorella sp. MSU3, respectively. A biomass of 2.33 g/L with lipid of 94.9 mg/L, 2.73 g/L with 398.9 mg/L, 5.28 g/L with lipid of 67.0 mg/L were found from Chlorella sp. MSU1, Chlorella sp. MSU2 and Chlorella sp. MSU3, respectively, under heterotrophic condition. Heterotrophic lipid production of microalgae Chlorella sp. MSU2 reached the maximum of 797.7 mg/L at 40 g/L glucose was obtained. Maximum cell yield coefficient (YX/S) was found of 0.733 using 20 g/L glucose, whereas volumetric lipid production rate (QP) of 132.9 mg/L/d was obtained using 40 g/L glucose.