| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 676466 | APCBEE Procedia | 2012 | 6 Pages |
Abstract
This work aims to establish characteristic SDS-PAGE profiles of peanut seed albumins and prolamins associated with Aspergillus flavus resistance assay. Comparing to the traditional method, a successful alternate method was developed by sequential extraction with 2 M NaCl in buffer (pH 6.8), 70% ethanol and w ter. The alcohol-soluble prolamin fraction from the present method showed unique profiles possessing antifungal activity against spore germination and mycelial growth. The water-soluble albumin fraction had specific profiles and demonstrated inhibition only on mycelial growth. Yet, the salt-soluble globulin fraction enhanced both processes of A. flavus propagation.
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