Cytotoxic Effect of Water, Ethanol and Ethyl Acetate Extract of Black Cincau (Mesona Palustris BL) Against HeLa Cell Culture

Black cincau (Mesona palustris BL) or grass black jelly as a traditional Indonesian food that has been used as a folk medicine and as a beverage for many centuries. It was reported that some of pharmaceutical effect of herbs could be related to their possible antioxidant activities. Moreover, some researchers reported that diverse compounds naturally occurring in food could exert an antimutagenic effect due to their antioxidant capacity. The studies to determine the bioactivity of black cincau extract against Hela cell. Cytotoxicity assays was conducted using method the MTT with 10 levels of extract concentration. The results of cytotoxicity assays showed the water extract, ethanol extract and the ethyl acetate extract of black cincau has anticancer activity against Hela cell lines. The third activity of black cincau extracts was significantly different. Water extracts of black cincau at low concentrations (19.53 - 156,25 μg/ml) showed higher anticancer activity than extracts ethanol and ethyl acetate. IC50 water extract of black cincau was 132,6 μg/ml, ethanol extract of black cincau was 146,0 μg/ml and ethyl acetate extract of black cincau was 182,96 μg/ml.