| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 677215 | Biomass and Bioenergy | 2012 | 4 Pages |
The screening for high lipid producing microalgae is a crucial step to enable large scale cultivation of suitable microalgae as a means of alternative energy. The Bligh and Dyer method has been traditionally used for the determination of biofuel-related lipids. But, we found that this method also extracts chlorophyll, to result in significant over-estimation of biofuel-related lipid measures. In this work, we present and characterize a method to obviate the chlorophyll interference to biofuel-related lipid determination.
► In algae, chlorophyll significantly contaminates biofuel-related lipid extracts. ► Chlorophyll contamination results in overestimation of biofuel-related lipids. ► An improvement to the Bligh and Dyer method was developed and characterized. ► DMSO was used to remove chlorophyll interference. ► DMSO does not affect biofuel-related lipid content.
