Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
679593 | Bioresource Technology | 2015 | 7 Pages |
Abstract
Sugars streams generated by organosolv pretreatment of hemp hurds, cellulose (C6) and hemicellulose (C5) fractions, were fermented to lactic acid (LA) by Bacillus coagulans strains XZL4 and DSM1. Pretreatment conditions and enzymatic hydrolysis were optimized and B. coagulans aptness to use lignocellulosic-derived sugars as a carbon source was evaluated. Methanolic organosolv pretreatment with 2.5% (w/w) H2SO4 gave the best results in terms of glucan recovery (98%), enzymatic hydrolysis of pretreated biomass (70%) and hemicellulosic sugars recovery (61%). C6 and C5 sugars fermentation by strain XZL4 gave, high LA yields (0.90 and 0.84Â g/g), high titers (141 and 109Â g/L), and high enantiomeric excess (>99%). Overall, 42Â g of l-LA were obtained from 100Â g of raw hemp hurds. These results can be considered promising for lignocellulosic feedstock valorization toward the production of polymer-grade LA.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Stefano Gandolfi, Lucia Pistone, Gianluca Ottolina, Ping Xu, Sergio Riva,