Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
679745 | Bioresource Technology | 2015 | 8 Pages |
Abstract
Polyhydroxyalkanoate (PHA) recovery from aerobic granules was investigated using four cell digestion agents, namely, sodium hypochlorite, sodium hydroxide, acetone and sodium chloride. Simultaneously, the removal of extracellular polymeric substances (EPS) and its effect on PHA yield were investigated. The highest PHA recovery yield was obtained using sodium hypochlorite, accounting for 89% cell dry weight (CDW). The highest PHA was recovered after the sodium hypochlorite completely removed the EPS from the aerobic granules. The average molecular weight (Mw) of the PHA recovered using sodium hypochlorite was 5.31Â ÃÂ 105Â g/mol with only 1.8% molecular weight degradation. The energy and duration analysis for PHA recovery revealed that the sodium hypochlorite method required the least amount of energy and time at 0.0561Â MJ/g PHA and 26Â h, respectively. The PHA that was recovered was a P3(HB-co-HV) co-polymer.
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Authors
K. Gobi, V.M. Vadivelu,