Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
679935 | Bioresource Technology | 2015 | 8 Pages |
•Bead milling can be used as a mild disintegration technique for microalgae.•Full cell disintegration can be achieved in less than 1 min.•Selective extraction of water soluble proteins is possible with bead milling.•The specific energy consumption can be kept below 2.5 kWh kgDW−1.
In this work, the mild disintegration of the microalgae Chlorella vulgaris for the release of intracellular products has been studied. By means of bead milling the microalgae suspensions were successfully disintegrated at different biomass concentrations (25–145 gDW kg−1) over a range of agitator speeds (6–12 m s−1). In all cases over 97% of cell disintegration was achieved resulting in a release of water soluble proteins. A clear optimum rate of disintegration and protein release was observed at an agitator speed of 9–10 m s−1 regardless of the biomass concentration. Selective extraction of water soluble proteins was observed as proteins released sooner than cell disintegration took place. Proteins could be released at 85% lower energy input than for cell disintegration resulting in specific energy consumptions well below 2.5 kWh kgDW−1.