| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 680078 | Bioresource Technology | 2015 | 7 Pages |
•A Brazilian Mucor circinelloides strain was found to be a good SCO producer.•The microwave-assisted EtOH extraction led to the best extraction of SCO.•The SCO showed considerable amount of monounsaturated fatty acids.•The enzymatic ethanolysis of the SCO achieved high yields of esters.•Biodiesel properties from SCO are comparable with those from vegetable oils.
The filamentous fungus Mucor circinelloides URM 4182 was tested to determine its ability to produce single-cell oil suitable for obtaining biodiesel. Cell growth and lipid accumulation were investigated in a medium containing glucose as the main carbon source. A microwave-assisted ethanol extraction technique (microwave power ⩽200 W, 50–60 °C) was established and applied to lipid extraction from the fungal hyphae to obtain high lipid concentration (44%wt) of the dry biomass, which was considerably higher than the quantity obtained by classical solvent methods. The lipid profile showed a considerable amount of oleic acid (39.3%wt), palmitic acid (22.2%wt) and γ-linoleic acid (10.8%wt). Biodiesel was produced by transesterification of the single-cell oil with ethanol using a immobilized lipase from Candida antarctica (Novozym® 435) as the catalyst. 1H NMR and HPLC analyses confirmed conversion of 93% of the single-cell oil from M. circinelloides into ethyl esters (FAEE).
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