| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 680096 | Bioresource Technology | 2015 | 12 Pages |
•Pilot process supports integrated PHA production with municipal wastewater treatment.•Municipal-wastewater-based feast–famine produced biomass with PHA storage capacity.•The biomass accumulated PHA from VFA-rich fermented sludge centrate produced at 42 °C.•Sludge centrate with excess nutrients yields increased PHA productivities.•Consistent performance under real variable wastewater and operating conditions.
A pilot-scale process was operated over 22 months at the Brussels North Wastewater Treatment Plant (WWTP) in order to evaluate polyhydroxyalkanoate (PHA) production integration with services of municipal wastewater and sludge management. Activated sludge was produced with PHA accumulation potential (PAP) by applying feast–famine selection while treating the readily biodegradable COD from influent wastewater (average removals of 70% COD, 60% CODsol, 24% nitrogen, and 46% phosphorus). The biomass PAP was evaluated to be in excess of 0.4 gPHA/gVSS. Batch fermentation of full-scale WWTP sludge at selected temperatures (35, 42 and 55 °C) produced centrate (6–9.4 gCODVFA/L) of consistent VFA composition, with optimal fermentation performance at 42 °C. Centrate was used to accumulate PHA up to 0.39 gPHA/gVSS. The centrate nutrients are a challenge to the accumulation process but producing a biomass with 0.5 gPHA/gVSS is considered to be realistically achievable within the typically available carbon flows at municipal waste management facilities.
