Pickering emulsion stabilized by lipase-containing periodic mesoporous organosilica particles: A robust biocatalyst system for biodiesel production

•Pickering emulsion stabilized by lipase-containing PMO was constructed firstly.•The Pickering emulsion system was used as biocatalyst for biodiesel production.•The Pickering emulsion showed outstanding activity, mechanical and storage stability.•Applications of this Pickering emulsion system in biocatalysis field are promising.

A novel catalytic system of Pickering emulsion stabilized by lipase-containing periodic mesoporous organosilica was constructed (named LP@PE) and used as biocatalyst for biodiesel production. The reaction parameters were optimized and the optimum conditions were as follows: the water fraction 0.65%, molar ratio of ethanol to oleic acid 2:1, immobilized lipase particles 150 mg, phosphate buffer pH 7.0 and temperature 30 °C. Under these conditions, the maximum biodiesel yield obtained via esterification of oleic acid with ethanol could reach 95.8%. The biodiesel yield could maintain 88.6% after LP@PE was used 15 times. The LP@PE was also used in the synthesis of biodiesel from Jatropha curcas oil. The highest yield could reach 87.1% and the yield was 73.0% after 10 cycles. All these results demonstrated that Pickering emulsion system stabilized by immobilized enzyme may possess much potential in many enzymatic industrial applications.