Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6810367 | Neurobiology of Aging | 2011 | 13 Pages |
Abstract
Aim of present study was to analyze the methylation pattern of PSEN1 promoter in SK-N-BE neuroblastoma cells and TgCRND8 mice, in a B vitamin (folate, B12 and B6) deficiency paradigm. PSEN1 methylation status has been evaluated through bisulphite modification and genomic sequencing. We demonstrate that B vitamin deficiency induces hypomethylation of specific CpG moieties in the 5â²-flanking region; S-adenosylmethionine has been supplemented as methyl donor to reverse this effect. PSEN1 promoter methylation status is correlated with gene expression. These findings pinpoint a direct relationship between B vitamin-dependent alteration of homocysteine cycle and DNA methylation and also indicate that PSEN1 promoter is regulated by methylation of specific CpG moieties.
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Authors
Andrea Fuso, Vincenzina Nicolia, Alessia Pasqualato, Maria Teresa Fiorenza, Rosaria A. Cavallaro, Sigfrido Scarpa,