Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
681154 | Bioresource Technology | 2013 | 5 Pages |
Highlight•A carbonyl reductase catalyzed NADPH-dependent reduction of DA to produce (3S)-AC.•A glucose dehydrogenase was coupled with the carbonyl reductase to produce (3S)-AC from DA.•(3S)-AC of 12.2 g l−1 was produced from DA of 14.3 g l−1 under optimal conditions.•The two-enzymes coupling system provided a potential pathway for (3S)-AC production.
Production of (3S)-acetoin ((3S)-AC), an important platform chemical, is desirable but difficult to perform. An NADPH-dependent carbonyl reductase (Gox0644) from Gluconobacter oxydans DSM 2003 was confirmed to have a good ability to reduce diacetyl (DA) to produce (3S)-AC. In this work, the NADPH-dependent carbonyl reductase was expressed and purified. Glucose dehydrogenase from Bacillus subtilis 168 was coupled with the NADPH-dependent carbonyl reductase to produce (3S)-AC from DA. Under the optimal conditions, 12.2 g l−1 (3S)-AC was produced from 14.3 g l−1 DA in 75 min. Because DA can be biotechnological produced, the two-enzymes coupling system might be a promising alternative for the (3S)-AC production.