Propionic acid production in glycerol/glucose co-fermentation by Propionibacterium freudenreichii subsp. shermanii

Propionibacterium freudenreichii subsp. shermanii can ferment glucose and glycerol to propionic acid with acetic and succinic acids as two by-products. Propionic acid production from glucose was relatively fast (0.19 g/L h) but gave low product yield (∼0.39 g/g) and selectivity (P/A: ∼2.6; P/S: ∼4.8). In contrast, glycerol with a more reduced state gave a high propionic acid yield (∼0.65 g/g) and selectivity (P/A: ∼31; P/S: ∼11) but low productivity (0.11 g/L h). On the other hand, co-fermentation of glycerol and glucose at an appropriate mass ratio gave both a high yield (0.54-0.65 g/g) and productivity (0.18-0.23 g/L h) with high product selectivity (P/A: ∼14; P/S: ∼10). The carbon flux distributions in the co-fermentation as affected by the ratio of glycerol/glucose were investigated. Finally, co-fermentation with cassava bagasse hydrolysate and crude glycerol in a fibrous-bed bioreactor was demonstrated, providing an efficient way for economic production of bio-based propionic acid.