| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 681208 | Bioresource Technology | 2013 | 5 Pages |
In this study we investigated the effect of starch amendment on α-glucosidase activity in an organic waste environment, treated under both mesophilic and thermophilic conditions. The relative effects of temperature on α-glucosidase activity with regard to in situ and assay conditions were investigated under a reciprocal design. The decline in dry matter under the different thermal regimes was consistent with the temperature independent degradation of starch. The results of extra-cellular enzyme analysis showed a significant relationship between starch addition and α-glucosidase activity, with evidence of thermal adaptation to the in situ temperature. A weaker, but significant, effect of starch addition on β-glucosidase activity was observed, with no evidence for thermal adaptation. Thus, our data is consistent with a substrate adaptive response to temperature, albeit under potentially a high selective pressure.
► Starch degradation was confirmed as temperature independent. ► α-glucosidase responded positively to starch addition. ► Evidence for α-glucosidase adaptation to in situ temperature.
