Enhancement of lipopeptides production in a two-temperature-stage process under SSF conditions and its bioprocess in the fermenter

A two-temperature-stage process was developed for the production of lipopeptides under SSF conditions. The effects of various temperatures, ranging from 25 to 40 °C, on the bacterial growth during the growth stage and on the production of lipopeptides during the productive stage were investigated. The optimum temperatures were found to be 30 °C for the growth of the strain and 37 °C for the biosynthesis of lipopeptides. The two-stage fermentation temperatures should be 30 °C in the initial 24 h and then 37 °C for the enhanced production of lipopeptides. The bioprocess results obtained in a 50 L fermenter verified the efficacy of this technique, which increased the yield of lipopeptides by 8.40% in flasks and by 13.11% in the fermenter, with a 4 h decrease of fermentation time in the fermenter. The 1000-fold scale-up of fermentation in a fermenter was successfully achieved.

► Effects of various temperatures on lipopeptides production was studied in SSF. ► A two temperature stage process was developed. ► The lipopeptides yields were enhancced by 8.40% in flask and 13.11% in fermenter. ► 1000-Fold scale-up fermentation in fermenter was successfully achieved.