Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
681350 | Bioresource Technology | 2012 | 7 Pages |
Microalgae are a promising feedstock for biofuels because of their capability to produce lipids. Cell disruption is necessary to maximize lipid extraction. Sonication conditions were evaluated for breaking heterotrophic (Schizochytrium limacinum) and autotrophic (Chlamydomonas reinhardtii) microalgae cells. Cell disruption was estimated by Nile red–lipids fluorescence quantification in S. limacinum and by the release of intracellular chlorophyll and carotenoids in green microalga C. reinhardtii. In both species, approximately 800 J/10 mL was the energy input necessary to maximize cell disruption, regardless of the cell concentrations studied. Increasing sonication time produced increasing amount of free radicals, quantified by the formation of hydroxyterephthalate. Sonication energy beyond the level needed for cell disruption induced oxidation of arachidonic acid, a polyunsaturated fatty acid typically found in marine lipids. Careful control of sonication conditions is necessary to maximize oil extraction at the lowest operational cost and to prevent oil from free radical-induced degradation.
► Ultrasonic algae cell disruption was monitored by intracellular material release. ► Lipid–Nile red fluorescence or cell pigments were used to quantify cell disruption. ► The energy input to maximize cell disruption was approximately 800 J/10 mL. ► Sonication at increasing energy inputs induced the formation of free radicals. ► The formation of lipid hydroperoxides is shown but more in-depth study is needed.