Article ID Journal Published Year Pages File Type
681545 Bioresource Technology 2012 6 Pages PDF
Abstract

A novel magnetically separable laccase immobilized system was constructed by adsorbing laccase into bimodal carbon-based mesoporous magnetic composites (CMMC). A large adsorption capacity (491.7 mg g−1), excellent activity recovery (91.0%) and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability was enhanced to a great extent and operational stability was increased to a certain extent. The shift of kinetic parameters indicated affinity change between enzyme and substrate. Application of the immobilized system in phenol and p-chlorophenol removal was investigated in a batch system. Adsorption effects of the support were responsible for the quick removal rate in the first hour, and up to 78% and 84% of phenol and p-chlorophenol were removed in the end of the reaction, respectively, indicating that the magnetic bimodal mesoporous carbon is a promising carrier for both immobilization of laccase and further application in phenol removal.

► A new laccase immobilized system was built using magnetic bimodal mesoporous carbon. ► Immobilized laccase exhibited excellent adsorption capacity and activity recovery. ► Immobilization improved reusability and resistance to pH and temperature changes. ► Nearly 80% of phenols were removed by the immobilized laccase in 12 h. ► Adsorption effects of the support mainly contributed to the first hour of removal.

Related Topics
Physical Sciences and Engineering Chemical Engineering Process Chemistry and Technology
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