Article ID Journal Published Year Pages File Type
681776 Bioresource Technology 2011 4 Pages PDF
Abstract

A mutant plant (Arabidopsis thaliana), sex1-1 (starch excess 1-1), accumulating high starch content in leaves was created to serve as better biomass feedstock for a H2-producing strain Clostridium butyricum CGS2, which efficiently utilizes starch for H2 production but cannot assimilate cellulosic materials. The starch content of the mutant plant increased to 10.67 mg/fresh weight, which is four times higher than that of wild type plant. Using sex1-1 mutant plant as feedstock, C. butyricum CGS2 could produce 490.4 ml/l of H2 with a H2 production rate of 32.9 ml/h/l. The H2 production performance appeared to increase with the increase in the concentration of mutant plant from 2.5 to 10 g/l. The highest H2 to plant biomass yield was nearly 49 ml/g for the mutant plant. This study successfully demonstrated the feasibility of using a starch-rich mutant plant for more effective bioH2 production with C. butyricum CGS2.

► We created a starch-rich Arabidopsis plant to serve as better feedstock for bioH2 production. ► The mutant plant contained 4-time higher starch content than that of wild-type plant. ► An indigenous isolate Clostridium butyricum CGS2 was used to efficiently produce bioH2 using the mutant plant at different feedstock loadings. ► This work demonstrated the feasibility of applying genetic modification techniques to improve the quality of feedstock for bioH2 production.

Related Topics
Physical Sciences and Engineering Chemical Engineering Process Chemistry and Technology
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