Substrate interactions during the biodegradation of BTEX and THF mixtures by Pseudomonas oleovorans DT4

► The substrate interactions during the aerobic biotransformation of THF and BTEX mixtures in P. oleovorans DT4. ► THF biodegradation afforded great energy for the biodegradation of BTEX. ► THF and BTEX degradation occurs primarily via two independent and inducible enzymes in P. oleovorans DT4. ► Two distinguished biodegradation pathways for benzene in the absence and presence of THF by P. oleovorans DT4.