Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
682196 | Bioresource Technology | 2010 | 4 Pages |
Abstract
A synthetic pathway consisting of octAB encoding succinyl-CoA:acetoacetate CoA-transferase and phaA encoding β-ketothiolase from Ralstonia eutropha H16 was constructed in Escherichia coli for extracellular production of acetoacetate. The recombinant E. coli produced 8.2 g/l acetoacetate in shake flasks at 37 °C in 24 h. In a 6-L fermentor, extracellular acetoacetate production was enhanced to 13.1 g/l after 30 h of fed-batch growth. To our knowledge, this is the first study on microbial production of acetoacetate.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Die Hu, Zhenyu Shi, Qiong Wu, Guo-Qiang Chen,