Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
682637 | Bioresource Technology | 2010 | 5 Pages |
Abstract
Paecilomyces bainier 229-7 was obtained after UV irradiation for 8 min in the presence of 0.4% LiCl and selection on potato dextrose media containing 30 mg/mL saponin from Panax notoginseng leaves (SPNL). The mutant produces ginsenoside Rd from ginsenoside Rb1 with a bioconversion rate as high as 94.9% under optimized culture conditions in shake flasks when supplied with 20 mg/mL of SPNL. Scale-up in 10-L fermenter resulted in an 89% bioconversion rate. Ginsenoside Rd was purified from the culture medium by a macroporous resin with a chromatographic purity of 92.6%. These results suggest that P. bainier 229-7 could be useful for the preparation of ginsenoside Rd in the pharmaceutical industry.
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Authors
Li Ye, Chao-Qun Zhou, Wei Zhou, Pei Zhou, Dao-Feng Chen, Xiao-Hui Liu, Xun-Long Shi, Mei-Qing Feng,