Article ID Journal Published Year Pages File Type
682705 Bioresource Technology 2010 7 Pages PDF
Abstract

Cytidine 5′-diphosphorylcholine (CDP-choline) was produced using a high efficiency ATP regeneration system and the Kennedy pathway in whole cells of Saccharomycescerevisiae As 2.398. Out of eight variables, KH2PO4, glycerol and (NH4)2SO4 were considered to be the most significant factors by response surface methodology including a Plackett–Burman design, path of steepest accent and central composite design. The optimum levels of the three variables were 20.13 g/L KH2PO4, 12.35 g/L glycerol and 0.49 g/L (NH4)2SO4, respectively. Energy utilization efficiency increased from 10.59% to 16.72% and choline chloride conversion yields increased from 12.35% to 42.78%. A high efficiency ATP regeneration system improves CDP-choline production.

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