Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
683107 | Bioresource Technology | 2010 | 9 Pages |
Abstract
Sulfate-reducing fluidized-bed bioreactor (FBR) fed with ethanol-lactate mixture was operated at 35 °C for 540 days to assess mine wastewater treatment, biological hydrogen sulfide production capacity and acetate oxidation kinetics. During the mine wastewater treatment period with synthetic wastewater, the sulfate reduction rate was 62 mmol SO42- Lâ1 dâ1 and Fe and Zn precipitation rates were 11 mmol Fe Lâ1 dâ1 and 1 mmol Zn Lâ1 dâ1. After this, the hydrogen sulfide production was optimized, resulting in sulfate reduction rate of 100 mmol SO42- Lâ1 dâ1 and H2S production rate of 73.2 mmol H2S Lâ1 dâ1. The limiting step in the H2S production was the rate of acetate oxidation, being 50 mmol acetate Lâ1 dâ1. Therefore, FBR batch assays were designed to determine the acetate oxidation kinetics, and following kinetic parameters were obtained: Km of 63 μmol Lâ1 and Vmax of 0.76 μmol acetate g VSSâ1 minâ1. The present study demonstrates high-rate hydrogen sulfide production and high-rate mine wastewater treatment with ethanol and lactate fed fluidized-bed bioreactor.
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Authors
Laura M. Nevatalo, Annukka E. Mäkinen, Anna H. Kaksonen, Jaakko A. Puhakka,