Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
683564 | Bioresource Technology | 2010 | 8 Pages |
Abstract
Chitinase (EC 3.2.1.14) is an enzyme with multiple industrial applications. These include bioconversion of chitin waste, a highly resistant and abundant biopolymer from crustacean food industry, into glucosamine and chito-oligosaccharide value-added products. This paper reports on the expression of endochitinase (ChiA) from Bacillus licheniformis strain DSM8785 in E. coli and characterization of the recombinant enzyme. Recombinant ChiA could efficiently convert colloidal chitin to N-acetyl glucosamine and chitobiose at pH 4.0, 6.0 and 9.0 at 50 °C and retained its activity up to 3 days under these conditions, suggesting that this enzyme is suitable for bioconversion of chitin waste.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Chomphunuch Songsiriritthigul, Sasithorn Lapboonrueng, Phornsiri Pechsrichuang, Puntarika Pesatcha, Montarop Yamabhai,