Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
683613 | Bioresource Technology | 2009 | 5 Pages |
Abstract
Response surface methodology (RSM) was applied to optimize culture conditions for the growth of Candida utilis with bamboo wastewater. A significant influence of initial pH, fermentation time and yeast extract on biomass of C. utilis was evaluated by Plackett–Burman design (PBD). These factors were further optimized using a central composite design (CCD) and RSM. A combination of initial pH 6.1, fermentation time 69 h and yeast extract 1.17 g/L was optimum for maximum biomass of C. utilis. A 1.7-fold enhancement of biomass of C. utilis was gained after optimization in shake-flask cultivation. The biomass of C. utilis reached 19.17 g/L in 3 L fermentor.
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Authors
Xin Li, Jia Ouyang, Yong Xu, Mu Chen, Xiangyang Song, Qiang Yong, Shiyuan Yu,