Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
684319 | Bioresource Technology | 2009 | 6 Pages |
Abstract
Urease immobilized on alginate was utilized to detect and quantify As3+ in aqueous solution. Urease from the seeds of pumpkin (vegetable waste) was purified to apparent homogeneity by heat treatment and gel filtration (Sephadex G-200). Further enzyme was entrapped in 3.5% alginate beads. Urea hydrolysis by enzyme revealed a clear dependence on the concentration and interaction time of As3+. The process variables effecting the quantitation of As3+ was investigated using central composite design with Minitab® 15 software. The predicted results were found in good agreement (R2Â =Â 96.71%) with experimental results indicating the applicability of proposed model. The multiple regression analysis and ANOVA showed that enzyme activity decreased with increase of As3+ concentration and interaction time. 3D plot and contour plot between As3+ concentration and interaction time was helpful to predict residual activity of enzyme for a particular As3+ at a particular time.
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Authors
Mahe Talat, Om Prakash, S.H. Hasan,