Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
684889 | Bioresource Technology | 2008 | 5 Pages |
Abstract
Statistical experimental designs were applied for the optimization of phytase production by a marine yeast Kodamaea ohmeri BG3 in a cost-effective oats medium. Using Plackett–Burman design, oats, ammonium sulfate and initial pH were identified as significant factors and these factors were subsequently optimized using a central composite design (CCD). The optimum variables that supported maximum enzyme activity were oats 1.0%, ammonium sulfate 2.3%, glucose 2.0%, NaCl 2.0% and initial pH 6.3. The validity of the optimized variables was verified in shake-flasks level. An overall 9-fold enhancement in phytase activity (62.0 → 575.5 U/ml) was attained due to the optimization.
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Authors
X.Y. Li, Z.Q. Liu, Z.M. Chi,