| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 692009 | Journal of the Taiwan Institute of Chemical Engineers | 2009 | 4 Pages |
Abstract
Penicillin G amidase (PGA) from Alcaligenes faecalis, which has useful properties for combinatorial biochemistry, is produced by a recombinant Escherichia coli using a constitutive expression system. Cells are cultured on a semi-defined medium in a 5-L fermentor using fed-batch technology. The fermentation yield is about 23,000Â U PGA activity/L broth.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Bin-Quan Gou, Ju Chu, Si-Liang Zhang, Yong-Hong Wang, Ying-Ping Zhuang, Hua Huang, Zhen Li, Zhong-Yi Yuan,