| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 69324 | Journal of Molecular Catalysis B: Enzymatic | 2016 | 4 Pages |
•Organic solvents can change conformational stability of tannase.•Enzymatic activity tightly correlates with the conformational stability.•Organic solvents with cyclic structure is harmful to the conformational stability.
The conformational stability of enzyme has a crucial effect on its catalytic performance. The effects of six organic solvents with different structures on the conformational stability of tannase were studied using Fourier transform infrared spectroscopy in this work. This results indicated that the cyclic structure of organic solvent plays a negative role in the conformational stability of tannase. The alkyl group of organic solvent has an interaction with the groups of oxygen and nitrogen of tannase, and the interaction goes against the conformational stability. The findings potentially provide a deep insight into the relationship between the biocatalytic activity and conformational stability of enzymes and push the study on the interaction of enzyme with organic solvent.
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