Hanging drop: An in vitro air toxic exposure model using human lung cells in 2D and 3D structures

Article ID	Journal	Published Year	Pages	File Type
6972241	Journal of Hazardous Materials	2013	10 Pages	PDF

Abstract

âº Validated for the first time a hanging drop (HD) air toxic exposure method for inhalation exposures effect studies. âº Applied a four compartment mass balance model to calculate cellular exposure levels. âº Compared A549 2-dimentional (2D) cell culture and 3D cell cultures benzene sensitivity. âº Carried out up to 20 days in vitro exposure studies. âº Validated A549 cell hanging drop toluene and xylene exposure EC50 value with published static apical air exposure method.

Keywords

ECM VOC EC50 Internal effect concentration SOM QSAR benzene Volatile organic compound Quantitative structure–activity relationship Hanging drop Lung cells Bioavailability Extracellular matrix IEC

Related Topics

Physical Sciences and Engineering Chemical Engineering Chemical Health and Safety

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Hanging drop: An in vitro air toxic exposure model using human lung cells in 2D and 3D structures

Authors

Faye F. Liu, Cheng Peng, Beate I. Escher, Emmanuelle Fantino, Cindy Giles, Stephen Were, Lesley Duffy, Jack C. Ng,