Article ID Journal Published Year Pages File Type
6981842 Colloids and Surfaces B: Biointerfaces 2015 7 Pages PDF
Abstract

- Recombinant fluorescent proteins with the LPETG/A tags were site-specifically immobilized on gold surfaces modified with a short peptide tag using Sortase A.
- Layers of recombinant mCherry and GFP were immobilized on the gold surface layer-by-layer using Streptococcus pyogenes and Staphylococcus aureus Sortase A enzymes.
- Fluorescent microscopy, Western blotting, and atomic force microscopy were used to confirm sortase-mediated protein ligation and immobilization on the gold surface.
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Physical Sciences and Engineering Chemical Engineering Colloid and Surface Chemistry
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