Article ID Journal Published Year Pages File Type
6983066 Colloids and Surfaces B: Biointerfaces 2014 8 Pages PDF
Abstract

- Diffusion hindrance in extracellular matrix (ECM) gels used in 3D cultures is investigated.
- A cost-effective and simple spectrophotometer-based diffusivity assay is introduced, and complemented with FRAP and AFM measurements.
- Diffusion hindrance is largely controlled by the enhanced ECM viscosity.
- Unassembled ECM macromolecules may control ECM viscosity.
- Dense (but not sparse) ECM gels are suitable tissue surrogates in terms of diffusion.
Keywords
Related Topics
Physical Sciences and Engineering Chemical Engineering Colloid and Surface Chemistry
Preview
A spectrophotometer-based diffusivity assay reveals that diffusion hindrance of small molecules in extracellular matrix gels used in 3D cultures is dominated by viscous effects
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