Fusion protein bilayer fabrication composed of recombinant azurin/cytochrome P450 by the sortase-mediated ligation method

- We developed the fusion protein-based bilayer fabrication method. For this reason, fusion protein was conjugated by.
- For making the fusion protein, the azurin and cytochrome P450 were redesigned to introduce the specific peptide sequences for enzyme mediated ligation.
- The proposed bilayer fabrication technique appears to be a good method to multiple protein layer construction for multiple detection biosensor applications.