Protein adsorption and covalent bonding to silicon nitride surfaces modified with organo-silanes: Comparison using AFM, angle-resolved XPS and multivariate ToF-SIMS analysis

Article ID	Journal	Published Year	Pages	File Type
6983843	Colloids and Surfaces B: Biointerfaces	2013	8 Pages	PDF

Abstract

- Protein immobilization compared for Si3N4 modified with amino- and epoxy-silanes.
- Correlated ToF-SIMS and angle-resolved XPS data yield protein surface coverage.
- Surface coverage is higher and develops faster for adsorption than covalent bonding.
- AFM height vs. surface coverage relations reflect distinct packing and conformation.

Keywords

Gamma globulins Physical adsorption principal component regression Time-of-Flight Secondary Ion Mass Spectrometry Covalent bonding

Related Topics

Physical Sciences and Engineering Chemical Engineering Colloid and Surface Chemistry

Preview

Protein adsorption and covalent bonding to silicon nitride surfaces modified with organo-silanes: Comparison using AFM, angle-resolved XPS and multivariate ToF-SIMS analysis

Authors

K. Awsiuk, A. Budkowski, A. Psarouli, P. Petrou, A. Bernasik, S. Kakabakos, J. Rysz, I. Raptis,