| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6984171 | Colloids and Surfaces B: Biointerfaces | 2012 | 7 Pages |
Abstract
⺠Single-step generation of collagen and poly-l-Lysine (PLL) micropatterns on a poly(ethylene glycol) (PEG) functionalized glass surface for cell based assays. ⺠The spotted protein micropatterns remain stable on the PEG surface even after extensive washing. ⺠NIH-3T3 fibroblasts attach to PLL and collagen micropatterns whereas primary rat hepatocytes adhered and spread only on collagen micropatterns. ⺠This differing affinity enables to create a well structured microscale co-culturing system: primary hepatocyte arrays surrounded by NIH-3T3 fibroblasts. ⺠Large scale formation of primary rat hepatocyte arrays was demonstrated. ⺠NIH-3T3 fibroblast single cell arrays were formed on PLL micropatterns.
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Physical Sciences and Engineering
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Authors
Alicja Zarowna-Dabrowska, Ekaterina O. McKenna, Maaike E. Schutte, Andrew Glidle, Li Chen, Carlos Cuestas-Ayllon, Damian Marshall, Andrew Pitt, Martin D. Dawson, Erdan Gu, Jon M. Cooper, Huabing Yin,