Polymeric biocatalytic membranes with immobilized thermostable phosphotriesterase

Results demonstrated that although free SsoPox W263F showed a higher performance up to 30 days it completely loses its activity after two months. On the contrary biocatalytic membranes showed an initial loss of activity in the first days (residual specific activity of about 54%when immobilized on/into NSG-PES and 5% when immobilized on/into PVDF) but any change in stability during more than 5 months. In the case of PVDF, the residual specific activity can be enhanced tuning the amount of immobilized SsoPox W263F, in fact it was demonstrated that there is a trade-off between amount of immobilized enzyme and catalytic performance.