Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7064204 | Biomass and Bioenergy | 2014 | 8 Pages |
Abstract
Softwood substrates are recalcitrant in enzymatic hydrolysis to reducing sugars, in part because of unproductive binding of enzymes on lignin. Interactions between polyethylene glycol (PEG) and a softwood substrate were characterised in order to estimate the PEG loadings required for effective blocking of unproductive enzyme binding. For the first time, the adsorption of Rhodamine labelled PEG (Mw 3400) on thermo-mechanically treated Pinus radiata fibres was characterised by fluorimetry, giving a best-fit binding constant of 68 L gâ1. This was considerably stronger than published binding constants of 6 L gâ1 for enzymes on lignin, accounting for the success of PEG as an additive. Glucose yields from enzymatic hydrolysis of the same substrate, when unlabelled PEG (Mw 4000) was used, were consistent with a PEG adsorption capacity of 0.01-0.02 g gâ1 substrate. Thus, PEG was shown to be effective at affordably low loadings and further mathematical modelling work predicts savings correspond to 6 kg PEG per tonne when pretreated Pinus radiata substrate was processed at higher substrate concentration.
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Authors
Alankar A. Vaidya, Roger H. Newman, Sylke H. Campion, Ian D. Suckling,