Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7065824 | Bioresource Technology | 2018 | 8 Pages |
Abstract
It was found that Corynebacterium glutamicum ÎiolR devoid of the transcriptional regulator IolR accumulates high amounts of d-xylonate when cultivated in the presence of d-xylose. Detailed analyses of constructed deletion mutants revealed that the putative myo-inositol 2-dehydrogenase IolG also acts as d-xylose dehydrogenase and is mainly responsible for d-xylonate oxidation in this organism. Process development for d-xylonate production was initiated by cultivating C. glutamicum ÎiolR on defined d-xylose/d-glucose mixtures under batch and fed-batch conditions. The resulting yield matched the theoretical maximum of 1â¯molâ¯molâ1 and high volumetric productivities of up to 4â¯gâ¯Lâ1â¯hâ1 could be achieved. Subsequently, a novel one-pot sequential hydrolysis and fermentation process based on optimized medium containing hydrolyzed sugarcane bagasse was developed. Cost-efficiency and abundance of second-generation substrates, good performance indicators, and enhanced market access using a non-recombinant strain open the perspective for a commercially viable bioprocess for d-xylonate production in the near future.
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Authors
Niklas Tenhaef, Christian Brüsseler, Andreas Radek, René Hilmes, Pornkamol Unrean, Jan Marienhagen, Stephan Noack,